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On TCBS agar, non-sucrose-fermenting Vibrio species typically produce which color colonies?

Yellow

Green

Non-sucrose-fermenting Vibrio species form green colonies on TCBS agar. This medium uses a pH indicator that turns yellow when acid is produced from sucrose fermentation; if a Vibrio doesn’t ferment sucrose, there’s no acidification around the colony, so the colonies retain the green color of the medium. This differential color change helps distinguish vibrios that ferment sucrose from those that don’t, aiding identification in clinical samples.

Blue

Orange

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