Which test is most appropriate for the presumptive identification of Prevotella melaninogenica?

Which test is most appropriate for the presumptive identification of Prevotella melaninogenica?

• A. Naglertest
• B. SPS sensitivity test
• C. Cytotoxin assay
• D. Fluorescence test

Answer: (D)

Prevotella melaninogenica is a black-pigmented anaerobe that often produces porphyrin pigments. The fluorescence test takes advantage of these pigments by shining UV light on the colony and looking for a characteristic fluorescence. This rapid signal helps distinguish Prevotella melaninogenica from other anaerobes and nonpigmented species, making it a useful presumptive identification method in routine culture work. Other options are related to different organisms or toxin activities and do not provide the quick pigment-based clue essential for this organism.

Prevotella melaninogenica is a black-pigmented anaerobe that often produces porphyrin pigments. The fluorescence test takes advantage of these pigments by shining UV light on the colony and looking for a characteristic fluorescence. This rapid signal helps distinguish Prevotella melaninogenica from other anaerobes and nonpigmented species, making it a useful presumptive identification method in routine culture work.

Other options are related to different organisms or toxin activities and do not provide the quick pigment-based clue essential for this organism.